Microplastics (MPs) are ubiquitous in all water systems. Wastewater treatment plants (WWTPs) play a significant role in the environmental pathway of MPs, and can offer valuable insight into the extent to which human activities may contribute to MPs in water systems. They can also be utilized to evaluate the effectiveness of current treatment processes in removing MPs. However, there remains an impediment in the pursuit of this knowledge due to inconsistencies present in current MPs sampling, processing, analysis, and reporting methodologies. The goal of the study is to address the inconsistencies present in research methodologies by examining the effect of sample volume, type of collection, and processing method on MPs’ characteristics and counts. Another goal is to investigate the impact that WWTPs have on MPs composition and load to the environment. The facilities selected for this study varied in service area, hydraulic capacity, treatment processes, and receiving streams. Samples are processed via wet peroxidation and a series of filtration. Fourier Transform Infrared spectroscopy enabled with Focal Plane Array and integrated with Purency MPs finder is used to quantify and identify MPs. Future outcomes will inform the adequacy of applied methodologies and a preliminary understanding of MPs' status from the selected facilities. For example, MP estimates from a wet weather sampling event demonstrate that the WWTP receives 166 MP particles/L and discharges 82 MP particles/L. A comparison of the influent and effluent values indicates that the facility removes MPs. These preliminary outcomes will help standardize a framework for future MPs studies.